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antibody against aspp2  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology antibody against aspp2
    Antibody Against Aspp2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antibody+against+aspp2/53BP2+Antibody/pm39113261-31-5-9
    Average 94 stars, based on 10 article reviews
    antibody against aspp2 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Formalin-fixed Paraffin-Embedded:

    Article Title: Downregulation of ASPP2 in pancreatic cancer cells contributes to increased resistance to gemcitabine through autophagy activation
    Article Snippet: .. The antibody against ASPP2 (Santa Cruz) was tested on sections from formalin-fixed paraffin-embedded pancreatic cancer and para-cancerous tissue samples. .. Images were obtained with a Zeiss AXIO Imager.

    other:

    Article Title: ASPP2 Upregulation as a Novel Approach to TGF-β2-Induced Proliferative Vitreoretinopathy In Vivo and In Vitro .
    Article Snippet: Purpose: Proliferative vitreoretinopathy (PVR) can cause blindness and the pathogenesis is unclear.. Transforming growth factor (TGF)-β-induced epithelial-mesenchymal transition (EMT) of RPE cells is vital.. P53 protein 2 (ASPP2) was previously reported to inhibit EMT in PVR rats, but the specific mechanism is unveiled.



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    A: The fluorescence intensity shows the efficacy of lentivirus transfection to ARPE-19 cells. Green fluorescence represents the GAFP in the scrambled-lentivirus and <t>ASPP2-lentivirus.</t> B: Representative band of Western blot shows the enhanced protein expression of ASPP2 in cells with ASPP2-lentivirus transfection for 72h. Repetitive bands of both scrambled-lentivirus and ASPP2-lentivirus transfection groups were shown for robust validation. β-actin is selected for internal reference. The relative ASPP2 protein expression in scrambled-lentivirus and ASPP2-lentivirus group normalized to β-actin. Data are depicted as the mean±SD, n=3. aP<0.01. Scrambled-lentivirus group is set to 100%.
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    Image Search Results


    A: The fluorescence intensity shows the efficacy of lentivirus transfection to ARPE-19 cells. Green fluorescence represents the GAFP in the scrambled-lentivirus and ASPP2-lentivirus. B: Representative band of Western blot shows the enhanced protein expression of ASPP2 in cells with ASPP2-lentivirus transfection for 72h. Repetitive bands of both scrambled-lentivirus and ASPP2-lentivirus transfection groups were shown for robust validation. β-actin is selected for internal reference. The relative ASPP2 protein expression in scrambled-lentivirus and ASPP2-lentivirus group normalized to β-actin. Data are depicted as the mean±SD, n=3. aP<0.01. Scrambled-lentivirus group is set to 100%.

    Journal: International Journal of Ophthalmology

    Article Title: Upregulation of ASPP2 expression alleviates the development of proliferative vitreoretinopathy in a rat model

    doi: 10.18240/ijo.2021.12.02

    Figure Lengend Snippet: A: The fluorescence intensity shows the efficacy of lentivirus transfection to ARPE-19 cells. Green fluorescence represents the GAFP in the scrambled-lentivirus and ASPP2-lentivirus. B: Representative band of Western blot shows the enhanced protein expression of ASPP2 in cells with ASPP2-lentivirus transfection for 72h. Repetitive bands of both scrambled-lentivirus and ASPP2-lentivirus transfection groups were shown for robust validation. β-actin is selected for internal reference. The relative ASPP2 protein expression in scrambled-lentivirus and ASPP2-lentivirus group normalized to β-actin. Data are depicted as the mean±SD, n=3. aP<0.01. Scrambled-lentivirus group is set to 100%.

    Article Snippet: The primary antibody used was polyclonal antibody against ASPP2 (sc-53861; Santa Cruz).

    Techniques: Fluorescence, Transfection, Western Blot, Expressing, Biomarker Discovery

    Fluorescence micrograph showing the ASPP2 expression (red) in ARPE-19 cells and cell nuclei are counterstained with DAPI (blue). The white arrows show the cell junctions. DAPI: 4,6-diamino-2-phenylindole. Scale bars: 20 µm.

    Journal: International Journal of Ophthalmology

    Article Title: Upregulation of ASPP2 expression alleviates the development of proliferative vitreoretinopathy in a rat model

    doi: 10.18240/ijo.2021.12.02

    Figure Lengend Snippet: Fluorescence micrograph showing the ASPP2 expression (red) in ARPE-19 cells and cell nuclei are counterstained with DAPI (blue). The white arrows show the cell junctions. DAPI: 4,6-diamino-2-phenylindole. Scale bars: 20 µm.

    Article Snippet: The primary antibody used was polyclonal antibody against ASPP2 (sc-53861; Santa Cruz).

    Techniques: Fluorescence, Expressing

    A: ARPE-19 cell viability is measured at 0, 12, 24, 48, 72, and 96h after transfection by CCK-8 assay; B: The viability of stable cell lines with ASPP2-lentivirus and scrambled-lentivirus transfection were detected respectively by CCK-8 at 0, 12, 24, 48, 72, and 96h. Data are the mean±SD, n=3 experiments. aP<0.05, bP<0.01.

    Journal: International Journal of Ophthalmology

    Article Title: Upregulation of ASPP2 expression alleviates the development of proliferative vitreoretinopathy in a rat model

    doi: 10.18240/ijo.2021.12.02

    Figure Lengend Snippet: A: ARPE-19 cell viability is measured at 0, 12, 24, 48, 72, and 96h after transfection by CCK-8 assay; B: The viability of stable cell lines with ASPP2-lentivirus and scrambled-lentivirus transfection were detected respectively by CCK-8 at 0, 12, 24, 48, 72, and 96h. Data are the mean±SD, n=3 experiments. aP<0.05, bP<0.01.

    Article Snippet: The primary antibody used was polyclonal antibody against ASPP2 (sc-53861; Santa Cruz).

    Techniques: Transfection, CCK-8 Assay, Stable Transfection

    PVR development in rats treated with ASPP2-lentivirus or scrambled-lentivirus

    Journal: International Journal of Ophthalmology

    Article Title: Upregulation of ASPP2 expression alleviates the development of proliferative vitreoretinopathy in a rat model

    doi: 10.18240/ijo.2021.12.02

    Figure Lengend Snippet: PVR development in rats treated with ASPP2-lentivirus or scrambled-lentivirus

    Article Snippet: The primary antibody used was polyclonal antibody against ASPP2 (sc-53861; Santa Cruz).

    Techniques:

    Represent fundus images of BN rats at the 14-day and 28-day follow up. The white arrows indicate fibrous proliferation, which is observed in both the ASPP2-lentivirus and scrambled-lentivirus group, but more pronounced in the scrambled-lentivirus group.

    Journal: International Journal of Ophthalmology

    Article Title: Upregulation of ASPP2 expression alleviates the development of proliferative vitreoretinopathy in a rat model

    doi: 10.18240/ijo.2021.12.02

    Figure Lengend Snippet: Represent fundus images of BN rats at the 14-day and 28-day follow up. The white arrows indicate fibrous proliferation, which is observed in both the ASPP2-lentivirus and scrambled-lentivirus group, but more pronounced in the scrambled-lentivirus group.

    Article Snippet: The primary antibody used was polyclonal antibody against ASPP2 (sc-53861; Santa Cruz).

    Techniques:

    Represent ERG results of experimental PVR at day 14 after the induction. Retinal functions are better reserved in rats of the ASPP2-lentivirus group compared to the scrambled-lentivirus group.

    Journal: International Journal of Ophthalmology

    Article Title: Upregulation of ASPP2 expression alleviates the development of proliferative vitreoretinopathy in a rat model

    doi: 10.18240/ijo.2021.12.02

    Figure Lengend Snippet: Represent ERG results of experimental PVR at day 14 after the induction. Retinal functions are better reserved in rats of the ASPP2-lentivirus group compared to the scrambled-lentivirus group.

    Article Snippet: The primary antibody used was polyclonal antibody against ASPP2 (sc-53861; Santa Cruz).

    Techniques:

    A wave and b wave amplitude of ERG in rats treated with ASPP2-lentivirus and scrambled-lentivirus

    Journal: International Journal of Ophthalmology

    Article Title: Upregulation of ASPP2 expression alleviates the development of proliferative vitreoretinopathy in a rat model

    doi: 10.18240/ijo.2021.12.02

    Figure Lengend Snippet: A wave and b wave amplitude of ERG in rats treated with ASPP2-lentivirus and scrambled-lentivirus

    Article Snippet: The primary antibody used was polyclonal antibody against ASPP2 (sc-53861; Santa Cruz).

    Techniques:

    A: Representative Western band showing decreased expression of alpha-SMA and LC3-II/I in retinas of rats in ASPP2-lentivirus group compared to scrambled-lentivirus group. GAPDH was used as loading control. B: Bar graph shows the relative protein expression level of alpha-SMA and LC3-II/I, normalized to GAPDH, respectively. Data are depicted as the mean±SD, n=3. aP<0.01. Scrambled-lentivirus group is set to 100%.

    Journal: International Journal of Ophthalmology

    Article Title: Upregulation of ASPP2 expression alleviates the development of proliferative vitreoretinopathy in a rat model

    doi: 10.18240/ijo.2021.12.02

    Figure Lengend Snippet: A: Representative Western band showing decreased expression of alpha-SMA and LC3-II/I in retinas of rats in ASPP2-lentivirus group compared to scrambled-lentivirus group. GAPDH was used as loading control. B: Bar graph shows the relative protein expression level of alpha-SMA and LC3-II/I, normalized to GAPDH, respectively. Data are depicted as the mean±SD, n=3. aP<0.01. Scrambled-lentivirus group is set to 100%.

    Article Snippet: The primary antibody used was polyclonal antibody against ASPP2 (sc-53861; Santa Cruz).

    Techniques: Western Blot, Expressing, Control